Вот мое решение
if __name__ == '__main__':
f = open('temp.txt','w+')
flag = False
with open('file.txt') as fp:
for line in fp:
for temp in f:
if temp == line:
flag = True
print('Found Match')
break
if flag == False:
f.write(line)
elif flag == True:
flag = False
f.seek(0)
f.close()