Вот как я это сделал:
[DllImport("kernel32.dll", CharSet = CharSet.Auto, SetLastError = true)]
internal static extern SafeFileHandle CreateFile(string lpFileName, int dwDesiredAccess, int dwShareMode, IntPtr securityAttrs, int dwCreationDisposition, int dwFlagsAndAttributes, IntPtr hTemplateFile);
затем позже
int dwFlagsAndAttributes = 0x40000000;
var portName = "COM5";
var isValid = SerialPort.GetPortNames().Any(x => string.Compare(x, portName, true) == 0);
if (!isValid)
throw new System.IO.IOException(string.Format("{0} port was not found", portName));
//Borrowed from Microsoft's Serial Port Open Method :)
SafeFileHandle hFile = CreateFile(@"\\.\" + portName, -1073741824, 0, IntPtr.Zero, 3, dwFlagsAndAttributes, IntPtr.Zero);
if (hFile.IsInvalid)
throw new System.IO.IOException(string.Format("{0} port is already open", portName));
hFile.Close();
using (var serialPort = new SerialPort(portName, 115200, Parity.None, 8, StopBits.One))
{
serialPort.Open();
}