Я пытался запустить bcl2fastq, но он сказал [error] No bcl2fastq found on path.
Заранее спасибо за любую помощь и предложение!
Best,
Yue
li@li-HP-Pavilion-Desktop-590-p0xxx:~$ bcl2fastq --help
BCL to FASTQ file converter
bcl2fastq v2.20.0.422
Copyright (c) 2007-2017 Illumina, Inc.
Usage:
bcl2fastq [options]
li@li-HP-Pavilion-Desktop-590-p0xxx:~$ cellranger mkfastq --id=tiny-bcl --run=cellranger-tiny-bcl-1.2.0 --csv=cellranger-tiny-bcl-simple-1.2.0.csv
/home/li/opt/cellranger-3.1.0/cellranger-cs/3.1.0/bin
cellranger mkfastq (3.1.0)
Copyright (c) 2019 10x Genomics, Inc. All rights reserved.
-------------------------------------------------------------------------------
Martian Runtime - '3.1.0-v3.2.3'
2020-02-11 20:16:39 [jobmngr] WARNING: configured to use 21GB of local memory, but only 19.0GB is currently available.
2020-02-11 20:16:39 [runtime] Reattaching in local mode.
Serving UI at http://li-HP-Pavilion-Desktop-590-p0xxx:39889?auth=ECJkP0tXgwBHrQwqlcZBN1UtFck0Of4F83ahq6jb0OU
2020-02-11 20:16:39 [runtime] (reset-partial) ID.tiny-bcl.MAKE_FASTQS_CS.MAKE_FASTQS.MAKE_FASTQS_PREFLIGHT.fork0.chnk0
2020-02-11 20:16:39 [runtime] (reset-partial) ID.tiny-bcl.MAKE_FASTQS_CS.MAKE_FASTQS.MAKE_FASTQS_PREFLIGHT_LOCAL.fork0.chnk0
2020-02-11 20:16:39 [runtime] Found orphaned local stage: ID.tiny-bcl.MAKE_FASTQS_CS.MAKE_FASTQS.MAKE_FASTQS_PREFLIGHT
2020-02-11 20:16:39 [runtime] Found orphaned local stage: ID.tiny-bcl.MAKE_FASTQS_CS.MAKE_FASTQS.MAKE_FASTQS_PREFLIGHT_LOCAL
[error] No bcl2fastq found on path. demux requires bcl2fastq v2.17 or greater for RTA version: 1.18.66.4