Как насчет этой опции?
# Importing Image from PIL package
from PIL import Image
# creating a image object
im = Image.open(r"C:\\your_path\\image.png")
px = im.load()
print (px[4, 4])
px[4, 4] = (0, 0, 0)
print (px[4, 4])
cordinate = x, y = 150, 59
# using getpixel method
print (im.getpixel(cordinate))
# now plot it
import matplotlib.pyplot as plt
plt.plot(pix[x,y])
plt.ylabel('some numbers')
plt.show()